Virginijus Siksnys - Selected Publications#

1. Mogila I, Kazlauskiene M, Valinskyte S, Tamulaitiene G, Tamulaitis G, Siksnys V. Genetic Dissection of the Type III-A CRISPR-Cas System Csm Complex Reveals Roles of Individual Subunits. Cell Rep. 2019, 26, 2753-2765.

2. Tamulaitiene G, Manakova E, Jovaisaite V, Tamulaitis G, Grazulis S, Bochtler M, Siksnys V. Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family. Nucleic Acids Res. 2019, 47, 997-1010.

3. Kazlauskiene M, Kostiuk G, Venclovas Č, Tamulaitis G, Siksnys V. A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems. Science. 2017 357, 605-609. [Highly cited (37 times) paper , accompanied by previews in Science and Nature, selected by Faculty 1000]]

4. Kazlauskiene M, Tamulaitis G, Kostiuk G, Venclovas Č, Siksnys V. Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition. Mol Cell. 2016, 62, 295-306 (cited 45 times) .

5. Karvelis T, Gasiunas G, Young J, Bigelyte G, Silanskas A, Cigan M, Siksnys V. Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements. Genome Biol. 2015, 16, 253 (cited 50 times).

6. Tamulaitis G. Kazlauskiene, M. Manakova, M. Venclovas, C. Nwokeoji, A.O. Dickman, M.J. Horvath, P. Siksnys V. Programmable RNA Shredding by the Type III-A CRISPR-Cas System of Streptococcus thermophilus. Mol Cell 2014, 56, 506-17 (cited 95 times).

7. Sinkunas T, Gasiunas G, Waghmare SP, Dickman MJ, Barrangou R, Horvath P, Siksnys V. In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus. EMBO J. 2013; 2, 385-94 (cited 121 times).

8. Gasiunas G, Barrangou R, Horvath P, Siksnys V. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci U S A. 2012, 109, E2579-86. {Highly cited (774 times) paper, selected by Faculty 1000}

9. Sinkunas T, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V. Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system. EMBO J. 2011, 30:1335-42 (cited 172 times).

10. Sapranauskas R, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V. The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res. 2011, 39, 9275-82. (cited 304 times, selected by Faculty 1000)