!!Virginijus Siksnys - Selected Publications
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1. Mogila I, Kazlauskiene M, Valinskyte S, Tamulaitiene G, Tamulaitis G, Siksnys V. Genetic Dissection of the Type III-A CRISPR-Cas System Csm Complex Reveals Roles of Individual Subunits. Cell Rep. 2019, 26, 2753-2765.\\
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2. Tamulaitiene G, Manakova E, Jovaisaite V, Tamulaitis G, Grazulis S, Bochtler M, Siksnys V. Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family. Nucleic Acids Res. 2019, 47, 997-1010.\\
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3. Kazlauskiene M, Kostiuk G, Venclovas Č, Tamulaitis G, Siksnys V. A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas systems. Science. 2017 357, 605-609. [[Highly cited (37 times) paper , accompanied by previews in Science and Nature, selected by Faculty 1000]]\\
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4. Kazlauskiene M, Tamulaitis G, Kostiuk G, Venclovas Č, Siksnys V. Spatiotemporal Control of Type III-A CRISPR-Cas Immunity: Coupling DNA Degradation with the Target RNA Recognition. Mol Cell. 2016, 62, 295-306 (cited 45 times) . \\
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5. Karvelis T, Gasiunas G, Young J, Bigelyte G, Silanskas A, Cigan M, Siksnys V. Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements. Genome Biol. 2015, 16, 253 (cited 50 times). \\
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6. Tamulaitis G. Kazlauskiene, M. Manakova, M. Venclovas, C. Nwokeoji, A.O. Dickman, M.J. Horvath, P. Siksnys V. Programmable RNA Shredding by the Type III-A CRISPR-Cas System of Streptococcus thermophilus. Mol Cell 2014, 56, 506-17 (cited 95 times). \\
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7. Sinkunas T, Gasiunas G, Waghmare SP, Dickman MJ, Barrangou R, Horvath P, Siksnys V. In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus. EMBO J. 2013; 2, 385-94 (cited 121 times). \\
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8. Gasiunas G, Barrangou R, Horvath P, Siksnys V. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci U S A. 2012, 109, E2579-86. {Highly cited (774 times) paper, selected by Faculty 1000}\\
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9. Sinkunas T, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V. Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system. EMBO J. 2011, 30:1335-42 (cited 172 times). \\
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10. Sapranauskas R, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V. The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res. 2011, 39, 9275-82. (cited 304 times, selected by Faculty 1000)