Wim Annaert - Selected Publications#

1. Annaert, W., Esselens, C., Baert, V., Boeve, C., Snellings, G., Cupers, P., Craessaerts, K., De Strooper, B. (2001). Interaction with telencephalin and the amyloid precursor protein predicts a ring structure for presenilins.

Neuron, 32(4), 579-89. IF: 14,377; #Citations: 174.

2. Esselens, C., Oorschot, V., Baert, V., Raemaekers, T., Spittaels, K., Serneels, L., Zheng, H., Saftig, P., De Strooper, B., Klumperman, J., Annaert, W. (2004). Presenilin 1 mediates the turnover of telencephalin in hippocampal neurons via an autophagic degradative pathway. Journal of Cell Biology, 166(7), 1041-54. IF: 10,822; #Citations: 148.

3. De Strooper, B., Annaert, W. (2010). Novel Research Horizons for Presenilins and γ-Secretases in Cell Biology and Disease. Annual Review of Cell and Developmental Biology, 10, 235-260. IF: 14,1; #Citations: 187.

4. Sannerud, R., Declerck, I., Peric, A., Raemaekers, T., Menendez, G., Zhou, L., Veerle, B., Coen, K., Munck, S., De Strooper, B., Schiavo, G., Annaert, W. (2011). ADP ribosylation factor 6 (ARF6) controls amyloid precursor protein (APP) processing by mediating the endosomal sorting of BACE1. PNAS, 108(34), E559-E568. IF: 9.7; #Citations: 162.

This paper shows for the first time the spatial separation of BACE1 vs APP, a limiting factor in processing.

5. Coen K, Flannagan RS, Baron S, Carraro-Lacroix LR, Wang, D, Vermeire W, Michiels C, Munck S, Baert V, Sugita S, Wuytack F, Hiesinger PR, Grinstein S, Annaert W. (2012) Lysosomal calcium homeostasis defects, not proton pump defects, cause endo-lysosomal dysfunction in presenilin-deficient cells. J. Cell Biology, Epub July 2; 198(1), 23-35. IF: 10,822; #Citations: 149.

This paper links PSEN function to lysosomal Ca2+ and not pH, and a start for the search of the mechanism.

6. Jurisch-Yaksi, N., Rose, A., Lu, H., Raemaekers, T., Munck, S., Baatsen, P., Baert, V., Vermeire, W., Scales, S., Verleyen, D., Vandepoel, R., Tylzanowski, P., Yaksi, E., de Ravel de l'Argentière, T., Yost, H., Froyen, G., Arrington, C., Annaert, W. (2013). Rer1p maintains ciliary length and signaling by regulating γ-secretase activity and Foxj1a levels. J. Cell Biology, 200(6), 709-720. IF: 10,822; #Citations: 23.

We demonstrate how overactivation of γ-secretase, through increased assembly causes a ciliopathy in vivo.

7. Peric, A., Annaert, W. (2015). Early etiology of Alzheimer’s disease: tipping the balance toward autophagy or endosomal dysfunction. Acta Neuropathologica, 129(3), 363-381. IF: 11,36; #Citations: 100.

8. Sannerud R, Esselens C, Ejsmont P, Mattera R, Rochin L, Tharkeshwar AK, De Baets G, De Wever V, Habets R, Baert V, Vermeire W, Michiels C, Grooth AJ, Wouters R, Dillen K, Vints K, Baatsen P, Munck S, Derua R, Waelkens E, Basi GS, Mercken M, Vooijs M, Bollen M, Schymkowitz J, Rousseau F, Bonifacino JS, Van Niel G, De Strooper B, Annaert W. (2016) Restricted location of PSEN2 γ-secretase determines substrate specificity and generates an intracellular Aβ pool. Cell, Jun 30;166(1):193-208. doi: 10.1016/j.cell.2016.05.020. Epub 2016 Jun 9. IF: 30,41; #citations: 179.

We discover here that γ-secretase heterogeneity is also reflected in the presence of distinct complexes in different compartments. This seminal paper laid the foundation for this grant proposal.

9. Van Acker Z.P., Bretou M., Annaert W. (2019) Endo-lysosomal dysregulations and late-onset Alzheimer's disease: impact of genetic risk factors. Mol Neurodegener. 14(1):20. doi: 10.1186/s13024-019-0323-7. E-pub: june 3th 2019. IF: 9,6; #Citations: 79.

10. Escamilla-Ayala, A.A., Sannerud, R., Mondin, M., Poersch, K., Vermeire, W., Paparelli, L., Berlage, C., Koenig, M., Chávez-Gutiérrez, L., Ulbrich, M.H., Munck, S., Mizuno, H., Annaert, W. (2020) Super-resolution microscopy reveals majorly mono- and dimeric presenilin1/γ-secretase complexes at the cell surface. doi: 10.7554/eLife.56679. IF: 8,14; #citations: 16.

We introduce single molecule resolution in the AD field and show unique features of single γ-secretase complexes in the bilayer of the plasma membrane. It introduces the imaging technologies for this proposal.